ASGSB 1998 Annual Meeting Abstracts

[58]
CO-LOCALIZATION OF ACTOMYOSIN AND CALRETICULIN AT AMYLOPLASTS PERIPHERIES: POSSIBLE IMPACTS FOR GRAVIPERCEPTION.   D. Volkmann, M. Pilger and F. Baluska. Botany Institute, University Bonn, Kirschallee 1, D-53115 BONN, Germany

Actin, myosin II and calreticulin have been localized at the peripheries of amyloplasts in different cell types of roots and shoots from maize by immunofluorescence microscopy. Sedimentable amyloplasts from the root cap statocytes showed prominent labelling for myosin whereas weaker labelling was observed for calreticulin, and actin. In non-sedimentable root cortex amyloplasts, labelling was prominent both for myosin as well for calreticulin, less so for actin. After osmotic stress, the labelling pattern had changed. In non-sedimentable amyloplasts of cortex cells, calreticulin decreased at the peripheries of amyloplasts and it was observed at pit fields located mainly at the longitudinal cell walls. The same situation was true to lesser extent for myosin. Actin labelling remained associated with the retracting protoplast of plasmolysed cells. These results indicate that the localization of prominent molecules related to the field of cytoskeleton and calcium is influenced by mechanical stress factors.

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