ASGSB 1998 Annual Meeting Abstracts

[88]
PHYTOHORMONES IN ASTROPLANTS BRASSICA RAPA, L.    L. Musatenko,¹ V. Generalova¹, V. Negretsky¹, N.Vedenicheva¹, L. Kadenyuk¹, and K. Sytnik¹. ¹Dept. of Phytohormonology, Inst. of Botany NASU, Kiev, Ukraine.

Studies on the effects of space flight factors on integral processes of plant growth and development require an investigation of phytohormones - compounds that affect cellular, tissue-specific and organismal interactions which are necessary for triggering and controlling the physiological and morphological programs. Research object - AstroPlants Brassica rapa L. grown in soil culture on earth for 9 and 15 days and for 9 days in microgravity condition (on the space shuttle Columbia STS-87, 1997).The phytohormones were investigated by HPLC, bioassay and MS/MS spectrometry. During studies of phytohormones at the various ontogenesis stages of B. rapa grown in earth conditions, we identified cytokinins - zeatin (Z), zeatin riboside (ZR), dihydrozeatin (DHZ); IAA, ABA and indolyleacetonitril (IAN), typical of Cruciferae, whose characteristics were obtained by qualitative analysis. As a result of studies on IAA, ABA and cytokinins in plants grown on orbit, it was shown that plants contain the hormonal complex typical of all higher plants. As far as chromatographic characteristics are concerned substances extracted from B. rapa are similar to standard solutions of corresponding phytohormones ( Sigma, USA). The phytohormones qualitative analysis give evidence for the presence of IAA, ABA, Z, ZR, zeatin glucoside (ZG), dihydrozeatin riboside (DHZR), isopenteniladenin (IP) and isopenteniladenosin (IPA). Thus, similarity in qualitative composition of identified components of phytohormone complex in B. rapa grown on earth and in space was shown. The obtained results testify to the promising prospects of conducting the studies on the regulation of plant growth and development in conditions of space flight. (Supported by NSAU)

Back to Meeting Program