ASGSB 1998 Annual Meeting Abstracts

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IDENTIFICATION OF TCH2 INTERACTING PROTEINS IN ARABIDOPSIS. W.-C. Chuang, K.A. Johnson, and J. Braam. Biochemistry and Cell Biology, Rice Univ., Houston.
The TCH2 gene of Arabidopsis thaliana is rapidly and transiently upregulated following a variety of exogenous stimuli, including touch, darkness, and temperature shifts. The TCH2 gene encodes a protein with 44% amino acid identity to Arabidopsis calmodulin proteins and has been demonstrated to bind calcium. Computer modeling of the primary TCH2 sequence (Khan et. al., 1997, Proteins 27:144) predicts that the tertiary structure is similar to calmodulin, but there are significant differences, including loss of acidic residues and other highly conserved residues, to suggest that the targets of TCH2 are unique from those of calmodulin. To understand the function of TCH2 in plant development and in response to exogenous stimuli, we are using a radioactively–labeled TCH2 fusion protein to identify cDNAs from an Arabidopsis expression library which encode TCH2 target proteins. The target proteins of initial interest will be those which interact with TCH2 in a calcium–dependent manner. (Supported by NASA Specialized Center for Research and Training, grant no. NAGW 5007.)

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