ASGSB-CSA-ELGRA
Joint Meeting

ASGSB SIXTEENTH ANNUAL MEETING
 October 25-28, 2000
Montreal, Canada

 PROGRAM

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WEDNESDAY, OCTOBER 25, 2000
15:00 Registration opens
19:00 Student Mixer
19:30 ASGSB Governing Board Meeting I
THURSDAY, OCTOBER 26, 2000
7:30 Registration Opens
SYMPOSIUM I

Consequences of Contamination of the Spacecraft Environment
8:30 - 8:45 Opening Remarks and Welcome
8:45 to 12:15  (Thursday, Oct. 26, 2000)
 Moderator: Richard Wassersug
TIME TITLE AUTHORS ABSTRACT # PAGE
8:45 Microbiological Contamination of Spacecraft Duane L. Pierson, R.J. Bruce, T.O. Groves, N.D. Novikova and A.N. Viktorov [1] 4
9:30 Contamination of Spacecraft Environment: Immunological Consequences William T. Shearer [2] 4
10:15 BREAK
10:45 Individual Variation in Human DNA Repair Genes: Consequences for the Space Traveler Barry W. Glickman, M. Khaidakov and A. Mortimer [3] 4
11:30 Plants, Plant Pathogens and Microgravity—A Deadly Trio Jan Leach [4] 4
12:30 LUNCH and COMMITTEE MEETINGS

CONCURRENT POSTERS I
A.  Space Life Sciences Training Program

Undergraduate Student Poster Competition

14:00 to 15:30 pm, (Thursday, Oct. 26, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
A01 A Novel Red-Light-Based Photosensory System That Mediates Positive Phototropism in Arabidopsis Roots

N.J. Ruppel, R.P. Hangarter and J.Z. Kiss.

 [5] 6
A03 Molecular Evolutionary Patterns in Microbial Natural Product Biosynthetic Gene and Enzyme Sequences: Search for Adaptive Significance H.E. Page, C.L. Peterson and J.V. Lopez [6] 6
A05 The Effects of Modified Biological Research in a Canister (Bric) Spaceflight Hardware on the Survivability and Development of the Tobacco Hornworm (Manduca sexta) M.R. Inzunza, K. Anderson and O. van den Ende [7] 6
A07 Synaptic Innervation in Rat Utricular Macula A. Chu and A. Lysakowski [8] 6
A09 Three-Dimensional Reconstruction and Analysis of Root Cap Statolith Distribution in Arabidopsis thaliana

R. Ehsanian, D.K. Bruck and J.D. Smith

 [9] 7
A11 Pesto Ground Control Experiments: A Gas Exchange System for Measuring Photosynthesis and Evapotranspiration

T. T. Tran, O. Monje and G.W. Stutte

 [10] 7
A13 Molecular Profiling of Planktonic and Biofilm Microbial Communities in Hydroponic Growth Systems E. Nunez, J.L. Adams and M.S. Roberts [11] 7
A15 The 16th Annual Spaceflight and Life Sciences Training Program at Kennedy Space Center, Florida

S. Potter, W. Hill, P. Currier, G. Koerner, J. Rebmann and A. Schlundt

 [12] 7
Graduate Student Poster Competition

2:00 to 3:30 pm, (Thursday, Oct. 26, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
B01 The Effect of Plastid Mutations on Gravitropism of Roots, Hypocotyls, and Inflorescence Stems of Arabidopsis K. Yamamoto and J. Z. Kiss [13] 9
B03 Gauging the Internal Gas Content of Brassica rapa Siliques Grown in Space K.L. Wilsen, J. Blasiak and M.E. Musgrave [14] 9
B05 Stress Response to Magnetic Levitation (Low-Gravity) and High Magnetic Fields in Transgenic Arabidopsis

A.N. Morgan, J. Yowtak, R.J. Ferl, J.S. Brooks, A.-L. Paul and M.W. Meisel

 [15] 9
B07 Changes in Osteoprogenitor Proliferation in the Rat Skeleton Due to Mechanical Unloading N. Basso, Y. Jia, C.G. Bellows and J.N.M. Heersche [16] 9
B09 ??? Tropism: When a Primary Root Encounters a Barrier to Downward Growth G.D. Massa and S. Gilroy [17] 10
B11 Rapid Discrimination among Individual DNA Molecules in Microliter Volumes W. Vercoutere, S. Winters-Hilt, H. Olsen, D. Deamer, D. Haussler and M. Akeson [18] 10
B13 Maternal Behaviour Under Hypergravity Conditions in CD-1 Mice M. Simeoni, D. Santucci and E. Alleva [19] 10
B15 Contribution of Sympathetic Activity to Lypolysis during Exposure to Increasing Hypergravity Loads M.M. Moran, T.P. Stein and C.E. Wade [20] 10
B17 Starch Conversion to Sucrose at Night S.E. Weise and T.D. Sharkey [21] 11

CONCURRENT POSTERS II
A.  Space Life Sciences Training Program

Undergraduate Student Poster Competition (continued)

15:30 to 17:00 pm, (Thursday, Oct. 26, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
A02 Magnetic Levitation as a Low Gravity Environment

J. Yowtak, A.N. Morgan, R.J. Ferl, J.S. Brooks, A.-L. Paul and M.W. Meisel

 [22] 13
A04 Molecular Mapping of the Lazy-2 Gravitropic Response Gene of Tomato J. Well, A. Madlung, K. Krutovskii, R. Meyer, T.J. White and T.L. Lomax [23] 13
A06 The Stability of Liquid Water in Porous Rocks in a Mars-like Environment C. Paty, C. McKay, D. Catling and J. Heldmann [24] 13
A8 Changes in Statocyte Structure and Amyloplast Starch in Arabidopsis  thaliana Columella Cells after Growth under Hypergravity Conditions S.D. Hopkins and J.D. Smith [25] 13
A10 Carbohydrate Deposition in Raphanus sativus L. cv. Cherry Belle Shoots:  Preliminary Ground Studies for the Rasta Spaceflight Experiment H.N. Goldsmith, E.C. Stryjewski, G.W. Stutte, W. McLamb and D. Reed [26] 14
A12 Soil Water Potential Affects Crop Growth Rate of Wheat Trough Changes in Leaf Area, Not  Photosynthesis H.-T. Wang, O. Monje and G.W. Stutte [27] 14
A14 Development of Defined Media for Rapid Physiological Profiling of Microbial Communities F.R. Perez, J.L. Garland and M.S. Roberts [28] 14
A16 SLSTP 2000, Controlled Biological Systems Group, Student Research Projects Support NASA’s Bioregenerative Life Support Program D. Muhlestein and G. Koerner [29] 14
Graduate Student Poster Competition (continued)

15:30 to 17:00 pm, (Thursday, Oct. 26, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
B02 Chimeric Calcium/Calmodulin-Dependent Protein Kinase: Role of the Neural Visininl-like Domain in Regulating Autophosphorylation and Calmodulin Affinity P.V.Sathyanarayanan, C.R.Cremo, W.F.Siems and B.W.Poovaiah [30] 16
B04 Development of the Nervous System and its Control of Gravity-Dependent Behaviours in Larvae of Bivalve Molluscs J.T. Plummer, D.L. Jackson and R.P. Croll [31] 16
B06

Innervation of Rat Vestibular Maculae in Hypergravity: an In Vivo and In Vitro Study

 S. Gaboyard, E.Scarfone, J. Lehouelleur and A. Sans [32] 16
B08 A Putative Role for the Cerebellum in Avian Vestibular Responses to Linear Translation S. Irons-Brown, S.M. Jones and T.A. Jones [33] 16
B10 Effects of Short-Duration Microgravity on Drosophila melanogaster (Fruit Fly) Activity M.S. Miller and T.S. Keller [34] 17
B12 Long-Term In Vivo Delivery of Recombinant Human Insulin-like Growth Factor-1 by Tissue-Engineered Skeletal Muscle Implants for Treating Disuse Muscle Atrophy in Mice

P.H.U. Lee, X.Y. Wang and H.H. Vandenburgh

 [35] 17
B14

Mechanical Stress and Wounding Elict Nitric Oxide Production in A. thaliana Wild-Type and Nitrate Reductase Mutants

H. Garcês, D.J. Durzan and M.C. Pedroso

 [36] 17
B16 Kinetics and Location of Phototropism in Zea mays L. Roots C. Wolverton, J.L. Mullen, H. Ishikawa and M.L. Evans [37] 17
B18 Video Capture of Green Fluorescent Protein Reporting In Vivo, Real Time Gene Expression M.S. Manak, A-L. Paul, P.C. Sehnke and R.J. Ferl [38] 18
19:00 Reception      
FRIDAY, October 27, 2000
SYMPOSIUM II

Psychological Issues In Long-Term Space Flight

9:00 to 12:30, (Friday, Oct. 27, 2000)
Moderator: Gerry Sonnenfeld
TIME TITLE AUTHORS ABSTRACT # PAGE
9:00 Psychosocial Issues in Long-Term Space Flight: Overview Larry Palinkas [39] 20
9:45 Psychosocial Issues in Space: Results from Shuttle/Mir.   Nick Kanas, V. Salnitskiy, E. Grund, D.S. Weiss, V. Gushin, O. Kozerenko, A. Sled and C.R. Marmar [41] 20
10:30 BREAK
11:00

Issues for the Future

 Gro Sandal

[42] 20
11:45 Intermission
12:00 The New Biology Enterprise at NASA Julie Swain None N/A
12:30 LUNCH and COMMITTEE MEETINGS

CONCURRENT ORAL SESSIONS I and II
ORAL SESSION I

ANIMAL DEVELOPMENT, PHYSIOLOGY
and GRAVITY SENSING

14:00 to 16:00, (Friday, Oct. 27, 2000)
Moderator: Michael Wiederhold
TIME TITLE AUTHORS ABSTRACT # PAGE
14:00 (Micro-)Gravity Actions as Identified from Developmental Biology Experiments in Space and Their Interpretation H.-J. Marthy [43] 22
14:15 The Effects of Microgravity on the Swimming Behaviour of Starfish Larvae B.J. Crawford and D.L. Jackson [44] 22
14:30 A Critical Period for Vestibular Development in Zebrafish (Danio rerio) S.J. Moorman, R. Cordova and S.A. Davies [45] 22
14:45 Functional Changes in Central Vestibular Relay Circuits Following 2g Centrifugation

S.M. Jones, L. Warren, R. Shukla, A. Browning, C.A. Fuller and T.A. Jones

 [46] 22
15:00 Tests to Determine the Adequacy of NASA’s Rodent Food Bars for Use in Long-Term Space Flight Experiments

J.E. Barrett, D.S. Yu and B.P. Dalton

 [47] 23
15:15 POMC and Endorphine Are Induced by Hypergravity in Rat Brain Y. Kumei, H. Shimokawa, R. Shimokawa, M. Terasawa,  B. Linsuwanont and K. Ohya [48] 23
15:30 Early and Late Effects of Perinatal Hyper-Gravity Exposure on the Developing CNS

E.M. Sajdel-Sulkowska, L.A. Baer, G.-H. Li, G.M. Sulkowski, A.E Ronca and C.E. Wade

 [49] 23
15:45 Gravitaxic Behavior in Drosophila melanogaster

J.D. Armstrong, M.J. Texada, E.L. Carter, E.S. Kuo, C.M. Nadorff and K.M. Beckingham

 [50] 23

CONCURRENT ORAL SESSIONS I and II
ORAL SESSION II

ADVANCED LIFE SUPPORT and BIOTECHNOLOGY

14:00 to 16:00, (Friday, Oct. 27, 2000)
Moderator:  Charles Barnes
TIME TITLE AUTHORS ABSTRACT # PAGE
14:00 Programmable Plants: Development of an In Planta System for the Remote Monitoring and Control of Plants for Long-Term Life Support C.S. Brown [51] 25
14:15 Boundary Layers around Plant Leaf and Root Tissues Depend on Gravity O. Monje, D.M. Porterfield and G.W. Stutte [52] 25
14:30 Genetic and Environmental Influences on the Nutritive Quality of Spinach: A NASA ALS Candidate Crop

C.F. Johnson, R.W. Langhans, L.D. Albright, R.M. Welch, G.F. Combs, R.P. Glahn and R.M. Wheeler

 [53] 25
14:45 Tissue Engineering in Zero Gravity A. Cogoli [54] 25
15:00 Use of the Rotating Bioreactor to Study Skeletal Mutations: Hereditary Multiple Exostosis P.J. Duke, D. Montufar-Solis and J.T. Hecht [55] 26
15:15 Fluid Handling and Management Experiment (FHAME)

T.M. Crabb, R.C. Morrow and T.K. Klemp

 [56] 26
15:30 Shear Forces and the Proper Centrol

J.J.W.A. van Loon, E. Folgering, J.P. Veldhuijzen and C.V.C. Bouten

 [57] 26
15:45 Advanced Versatile Tools for Life Sciences Research in Space

P. Todd, J. Vellinger, A. Sharpe, R. Ormsby, H. Platt, K. Barton and M. Deuser

 [58] 26

CONCURRENT POSTERS III
C.  ANIMAL DEVELOPMENT, PHYSIOLOGY and GRAVITY SENSING

16:00 to 17:30 pm, (Friday, Oct. 27, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
C01

Microgravity Effects on Fertilized Eggs Have No Incidence, after Landing, on the Further Larval Development and Reproduction in the Amphibian Pleurodeles Waltl

H. Membre, A. Bautz, D. Durand, C. Aimar, A.M. Bautz and C. Dournon

 [59] 28
C03 Microgravity-Induced Malformations of the Body Correlate with the Depression of the Static Vestibuloocular Reflex E. Horn [60] 28
C05 Gravity Stimulation Changes Withdrawal Reflex in Rats

K. Toda, Y. Kawauchi, Y. Kumei, F.H. Nasution and K. Makita

 [61] 28
C07 Hindlimb-Suspension, Water Deprivation and Salt-Loading Affect Angiotensin-Converting Enzyme (Ace) Expression in Rat Choroid Plexus E. Vila-Porcile, C. Carcenac, C. Masseguin, J.-M. Gasc and J. Gabrion [62] 28
C09 Lack of Vestibular Otolith Participation in Human Orthostatic Blood Pressure Control

D.E. Watenpaugh, A. Cothron, S.L. Wasmund, W.L. Wasmund, R. Carter III, N.K. Muenter and M.L. Smith

 [63] 29
C11 Regional and Muscle Specific Effects of a ß-Adrenergic Agonist in Hindlimb Suspended Rats

D.A. von Deutsch, I.K. Abukhalaf, L.E. Wineski, S.A. Pitts, R. Roper, L.D. Kataria, D.C. Jackson, D.E. Potter and D.F. Paulsen

 [64] 29
D.  CELL BIOLOGY

16:00 to 17:30 pm, (Friday, Oct. 27, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
D01 Vitamin D Production in the Rotating Wall Vessel (RWV)

F. Lewis, E.N. Benes, X.-C. Wang, P.L. Allen, T.G. Hammond and L.A. Cubano

 [65] 31
D03 L-Nmma Suppresses Nitric Oxide Production and Apoptosis in Taxus brevifolia Cells M.C. Pedroso and D.J. Durzan [66] 31
D05 Altered Gravity Increases PGE2 Production through Activation of COX-2 mRNA Expression in Mouse Osteoblast Like MC3T3-E1 Cells

A. Sato, M. Fujita, M. Kanematsu, M. Narato, H. Kumagai, S. Kamigaichi and M. Takaoki

 [67] 31
D07 The Effect of Microgravity on Cytoskeleton Architecture and Proliferation of Human Breast Cancer Cell Line MCF-7 J. Vassy, S. Portet, D. Schoevaert, M. Beil, G. Millot, G. Gasset and F. Fauvel-Lafève [68] 31
D09 Clinostat Rotation Culture Modulates Gene Expression of Osteoclastogenesis-Regulating Factors via a Cyclic-Amp Dependent Mechanism M. Kanematsu, H. Takai, M. Takaoki and A. Sato [69] 32
D11 Human Osteoblast Differentiation Is Expedited in Culture in a Magnetic Field L. Yuge, T. Kumagai, I. Hide, S. Hiyama, M. Kanno, Y. Kumei, S. Takeda,  Y. Ikuta,  M. Sugiyama and  K. Kataoka [70] 32
D13 Gravity Sensitivity of T-Cell Activation: The Actin Cytoskeleton B.B. Hashemi, J.E. McClure and D.L. Pierson [71] 32
D15 Development of a Microgravity Cell Culture Platform for the Study of Bone Cell Metabolism onboard the NASA Shuttle L. Misener, D. Sindrey, T. Smith, S. Pugh, D. Kusljic and P. Kwong [71A] 32
E.  PLANT DEVELOPMENT, PHYSIOLOGY and GRAVITY SENSING

16:00 to 17:30 pm, (Friday, Oct. 27, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
E01 Oxygen Effects on Pollen Germination and Tube Orientation

J. Blasiak, D. Mulcahy and M. Musgrave

 [72] 34
E03 Changes in Cotyledon Cell Ultrastructure during Brassica rapa Seed Development in Microgravity A. Kuang and M. E. Musgrave [73] 34
E05 Effects of Lithium Ions on Elongation and Gravitropic Responses of Primary Roots of Maize T. J. Mulkey [74] 34
E07 The Actin Network in Lentil Root Statocytes

D. Driss-Ecole and G. Perbal

 [75] 34
E09 Microscopic Analysis of Sweetpotato Root Tips Propagated from Stem Cuttings Maintained in Either Vertical or Horizontal Clinorotation

C.S. Williams, D.G. Mortley, C.E. Morris, C.F. Davis, S.D. Gamble and J.W. Williams

 [76] 35
E11 Ultrastructure of P. patens Tip Caulonemata Cells: Untreated, Cold Grown, Severed, Oryzalin Treated, UV-A Treated

E. B. Tucker, V. Sookhdeo and L Yin

 [77] 35
E13 The rib1 Mutant Is Resistant to Indole-3-Butyric Acid, an Endogenous Auxin in Arabidopsis Thaliana C.S. Waddell and J. Poupart [78] 35
F.  ADVANCED LIFE SUPPORT and BIOTECHNOLOGY

16:00 to 17:30 pm, (Friday, Oct. 27, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
F01 Regenerable Seed Plugs from Formed Plant Fiber

R.C. Morrow, C.J. Ehle and T.M. Crabb

 [79] 37
F03 Evapotranspiration by Salad Crops in Controlled Environments D.E. Ciolkosz and G.D. Goins [80] 37
F05 Astrium – A New Name in Space Life Sciences

P. Kern and U.M. Kuebler

 [81] 37
F07 IBIS: An Instrument Dedicated to Perform Biological Experiments in Microgravity D. Thierion, G. Gasset, D. Chaput, A. Labarthe, B. Eche and M. Viso [82] 37
G.  SPACEFLIGHT EXPERIMENT RESULTS

16:00 to 17:30 pm, (Friday, Oct. 27, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
G01 Expression of Fas/CD95 in Spaceflown Lymphocytes (Jurkat) L.A. Cubano and M.L. Lewis [83] 39
G03 Are There Two Mechanisms Underlying Space Motion Sickness? D.G.D. Watt [84] 39
G05 Payload Late Access Survey Preliminary Results C. Martin-Brennan and R.C. Morrow [85] 39
G07 Effect of Microgravity on Root Regeneration, Ultrastructures, and Carbohydrate Content of Sweetpotato Stem Cuttings

D.G. Mortley, C.S. Williams, C.F. Davis and J.W. Williams

 [86] 39
H.  SPACEFLIGHT PHYSIOLOGY and MEDICINE

16:00 to 17:30 pm, (Friday, Oct. 27, 2000)
NOTE: Presenters are to be next to their posters the entire time.
POSTER # TITLE AUTHORS ABSTRACT # PAGE
H01 Long-Term Effects of Microgravity on Human Sleep, Cytokine, and Endocrines