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ASGSB 2006 Annual Meeting Abstracts
[77]
Three
cyanobacteria (Anabaena sp., Plectonema
boryanum, Chroococcidiopsis
CCMEE171) and an alga (Chlorella
ellipsoidea) were grown under simulated martian ecopoesis
conditions. A xenon arc lamp with a solar
filter provided
simulated martian sunlight, and temperature cycled diurnally from
-80°C to
26°C. A Mars-like atmosphere of 100% CO2
was provided at 25, 100, 300, 500 and 1000 mbar. The
cyanobacteria and alga were inoculated
into JSC Mars-1 soil simulant and exposed to each atmospheric pressure
for five
weeks. Survival and growth were
determined via extractable chlorophyll a
and total esterase (fluorescein diacetate hydrolysis) activity. Maximum survival occurred at 100-300
mbar. At 25, 500 and 1000 mbar, esterase
activity was near zero, and extractable chlorophyll a
was less than 10% of control samples. Overall,
the cyanobacteria survived better
than the alga. Low survival at 25 mbar
was probably due to desiccation. Low
survival at 500 and 1000 mbar may have been due to CO2
toxicity. This research was supported by
the NASA Institute for Advanced Concepts and by the Arkansas Space
Grant
Consortium.
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